Is hard to see a lot of the characters being hit by the arrow, like Polnareff, or Kakyoin. since they both had stands since childhood.
Also, in part IV, when hit by an arrow, Koichi actually got a wound, and developed a stand, but a lot of stand users in part IV, when hit by the arrow, get an empty hole. But in part V, the arrow is able to hurt again.
Also, in part VI, Jolyne needed an piece of arrow do develop a stand, even being daughter of Jotaro. But, the sons of dio, were born after dio got his stand, and yet they were born with a stand, also, the stand spread to all the other members of the joestar bloodline that were already born in that time got their stands, but Holy lost her stand due do dio's defeat, but Josuke kept his, even if both stands were killing the user.